Fig. 6

SPAG5 triggers PI3K/AKT pathway. a Proteins from stable cell lines were subjected to western blot to examine the expression of phosphorylated AKT (p-AKT), AKT, phosphorylated ERK1/2 (p-ERK1/2) and ERK1/2. b Cells with SPAG5 overexpression were incubated with wortmanin (a specific inhibitor of PI3K) for 24 h. The cell proliferation was examined by foci formation assays. c The impact of activation of PI3K/AKT pathway in SPAG5-mediated cell migration was determined by Transwell assays. d Cells expressing SPAG5 were transfected with CEP55 siRNA for 24 h. The expression of p-AKT was examined by western blot. e A schematic diagram of SPAG5-promted cell growth and metastasis was shown. All *P < 0.05