Fig. 2
From: LncRNA DLEU1 contributes to colorectal cancer progression via activation of KPNA3
DLEU1 knockdown inhibits cell proliferation, migration and invasion in CRC. a Total RNAs were extracted from CRC cell lines (SW620, DLD-1, HCT8, RKO, CaCo2, HCT116, HT29 and SW480) and human colon cell line (CCD18-Co, FHC and HCoEpiC). Then the expression of DLEU1 was analyzed by RT-qPCR. b DLEU1 was successfully knocked down in HCT8 and SW480 cells. c DLEU1 knockdown dramatically inhibited cell proliferation. MTT assays were performed with shCtrl and shDLEU1 HCT8 or SW480 cells. Cell viabilities were measured at different time points. d DLEU1 knockdown remarkably impaired the ability of colony formation. e DLEU1 depletion decreased cell division. shCtrl and shDLEU1 HCT8 or SW480 cells were cultured in the presence of BrdU for 6 h. Then cells were collected and the incorporation of BrdU was analyzed by FACS. f DLEU1 knockdown greatly promoted cell death in HCT8 and SW480 cells. Cell apoptosis was evaluated by staining with active CASPASE 3 and analysis with FACS. g, h DLEU1 depletion inhibited cell migration and invasion. Scale bar, 50 μm. *P<0.05 and **P < 0.01. All data were collected from three independent experiments