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Fig. 6 | Molecular Cancer

Fig. 6

From: LncRNA DLEU1 contributes to colorectal cancer progression via activation of KPNA3

Fig. 6

DLEU1 promotes KPNA3 expression by recruiting SMARCA1 in CRC. a DLEU1 knockdown impaired the expression of KPNA3 in HCT8 and SW480 cells. The expression levels of neighboring genes of DLEU1 were measured by RT-qPCR after DLEU1 knockdwon. b DLEU1 depletion decreased the protein levels of KPNA3 in HCT8 and SW480 cells. c DLEU1 enriched on the promoter (− 800 bp~ − 250 bp from TSS) of KPNA3 in HCT8 and SW480 cells. d DLEU1 knockdown inhibited the enrichment of H3K27Ac on KPNA3 promoter in HCT8 and SW480 cells. e SMARCA1 enriched on KPNA3 promoter in HCT8 and SW480 cells. f DLEU1 knockdown impaired the enrichment of SMARCA1 on KPNA3 promoter in HCT8 and SW480 cells. g DLEU1 overexpression promoted the enrichment of SMARCA1 on KPNA3 promoter in HCT8 and SW480 cells. h SMARCA1 co-localized with KPNA3 promoter in HCT8 cells while DLEU1 knockdown abrogated it. Green, KPNA3 promoter probe; Red, SMARCA1; Blue, DAPI. Scale bar, 10 μm. i SMARCA1 silence decreased the expression of KPNA3 in HCT8 and SW480 cells while SMARCA1 overexpression up-regulated that of KPNA3. j Higher expression of KPNA3 in SMARCA1 highly expressed CC samples. Scale bar, 100μm. k Overexpressing DLEU1 promoted KPNA3 expression while deletion of nt 1~ 400 abrogated it in HCT8 and SW480 cells. **P<0.01 and ***P<0.001. All data presented are shown as means ± SD collected from three independent experiments

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