Fig. 1

Error rate reduction in LC103 and L82 gene panels compared to conventional NGS for Q30 bases. LC103 targets 103 regions of interest in 22 lung and colon cancer related genes. L82 interrogates 82 regions in 17 overlapping genes with LC103. Data were generated on Illumina NextSeq. Only the overlapping bases between two panels are plotted. At each base position, error rates are calculated by dividing the number of error alterations by the total base coverage using the data from cell line FFPE references after removing known mutations, and from healthy individuals analyzed for cfDNA analysis. The error rate of LC103 is well below 1%, which allows reliable mutation detection above mutant allele frequency (MAF) of 2% in tumor tissue. For all mutations in the Multiplex Reference standards (FFPE DNA or sections, Horizon Discovery), the observed allele frequencies are consistent with the expected allele frequencies. In the L82 dataset (green), recurrent background errors (< 0.1% of error rate) are shown in the figure. These errors appear at non-hotspot positions and can be further reduced using a position-specific unbiased approach