Fig. 1

The expression of BCRC-3 in cell lines and tissues, and the subcellular location of BCRC-3. a qRT-PCR assay with divergent primers indicated the low expression of BCRC-3 in 47 pairs of human BC compared with their adjacent normal tissues. b The expression of BCRC-3 in SV-HUC-1, EJ and T24 T cell lines were measured by qRT-PCR. c Schematic diagram demonstrated that nine exons derived from PSMD1 constituted BCRC-3. The existence of BCRC-3 was proved by RT–PCR and its back splicing junction was verified by Sanger sequencing. Red arrow indicated the special splicing junction of BCRC-3. d RT-PCR assay with divergent or convergent primers indicating the existence of BCRC-3 in SV-HUC-1, EJ, T24 T cell lines and three BC tissues. GAPDH was used as negative control. e qRT-PCR analysis of the expression of BCRC-3 after RNase R treatmet in EJ or T24 T cells. f RNA-FISH indicated the location of BCRC-3 in T24 T cells. U6 was used as negative control and 18S was used as positive control. Nuclei were stained blue by DAPI. BCRC-3, U6 and 18S were stained red with cy3 (scale bar, 10 μm). (Data are mean ± SEM of three experiments. Student’s t-test analyzed the difference in a-b, e. * P < 0.01 vs. normal bladder tissues, SV-HUC-1, or mock)