Fig. 2
From: Regulation of Sox2 and stemness by nicotine and electronic-cigarettes in non-small cell lung cancer
a An immunofluorescence experiment showing the induction of Sox2 by nicotine and E-cigarette extracts in A549 cells (top panels) and human mesenchymal stem cells (bottom panel). b. The induction of Sox2 by the same agents as seen by western blotting. c An RT-PCR experiment shows the induction of Sox2 by nicotine and E-cigarette extracts occurring at the transcriptional level in A549 cells. d Treatment of A549 and H1650 cells that are transiently transfected with a Sox2-Luciferase reporter with nicotine and E-cigarette extracts induces promoter activity, confirming the induction of Sox2 at the transcriptional level. e A western blot showing the induction of Sox2 protein by nicotine from 18 h to 48 h post treatment (f) A RT-PCR experiment showing the induction of Sox2 message at the same time points. The graphical data represented in this figure has ± standard deviation (SD) values derived from three independent experiments. The statistical comparisons between the groups were carried out by unpaired two tailed Student’s t-test