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Fig. 3 | Molecular Cancer

Fig. 3

From: Sunitinib-suppressed miR-452-5p facilitates renal cancer cell invasion and metastasis through modulating SMAD4/SMAD7 signals

Fig. 3

miR-452-5p level is maximized by P65 through directly binding its promoter. a The schematic illustration of the proposed model depicting of 4 putative upstream transcription factors of miR-452-5p from 3 prediction databases, Promo, TargetScan and miTar. b Bioinformatic analysis of potential P65 binding sites in miR-452-5p promoter. c Lysates of OSRC-2 cells were subjected to ChIP assay. d The schematic illustration of P65-wt/mut in the miR-452-5p promoter. e Luciferase reporter assays in OSRC-2 cells. f the correlation between miR-452-5p expression and corresponding P65 expression in RCC and normal renal tissues from TCGA RCC dataset. g P65 expression in RCC and normal renal tissues from TCGA RCC dataset. h IHC staining revealed the level of P65 expression in RCC tissues (×200, × 400) versus adjacent normal tissues. i P65 expression in different TNM stage of RCC samples from TCGA RCC dataset. j P65 expression and patients’ survival time from TCGA RCC dataset. Data shown are mean±S.D. *p<0.05

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Molecular Cancer

ISSN: 1476-4598