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Fig. 5 | Molecular Cancer

Fig. 5

From: Sunitinib-suppressed miR-452-5p facilitates renal cancer cell invasion and metastasis through modulating SMAD4/SMAD7 signals

Fig. 5

SMAD4 recapitulates the effects of miR-452-5p in RCC cells. a GSEA of published gene array, GO:0006810, GO:0001822 and GO:0000398 dataset referred to migration, invasion and metastasis related-gene signatures, respectively. NES, normalized enrichment score. b Cell migration in OSRC-2 and SW839 cells were analyzed by wound-healing assay. Cells were respectively transfected with vector, miR-452-5p, oe-SMAD4 and miR-452-5p+oe-SMAD4. c Cell invasion in OSRC-2 and SW839 cells were analyzed by transwell assays. Cells were respectively transfected with vector, miR-452-5p, oe-SMAD4 and miR-452-5p+oe-SMAD4. d The protein levels of SMAD4 and SMAD7 in OSRC-2 and SW839 cells were assessed by Western blot analysis. Respectively, cells were transfected with vector, miR-452-5p, oe-SMAD4 and miR-452-5p+oe-SMAD4 for 48 hours before detection. Data shown are mean±S.D. *p<0.05, **p<0.01

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Molecular Cancer

ISSN: 1476-4598