Fig. 6

miR-452-5p promotes RCC metastasis through altering SMAD4 in vivo. a Representative images of mice viewed by IVIS system in sh-miR-452-5p and sh-miR-NC group 2 weeks after left renal capsule injections (n=8). b Representative images of abdominal metastasis viewed by IVIS system in each group 4 weeks after the orthotopic xenograft transplantation (n= 8). c Presented are number of metastasis nodules in nude mice of sh-miR-452-5p and sh-miR-NC group. d The animals were euthanized 8 weeks later for primary RCC and metastases detection by bioluminescent imaging, gross examination, and histological staining with haematoxylin and eosin (H & E). Quantitations were also shown. e Representatives images of IHC staining of SMAD4 and SMAD7 inside or surrounding primary tumors, respectively. f-h Orthotopic xenograft animal models were also generated using mock, miR-452-5p, oe-SMAD4 and miR-452-5p+ oe-SMAD4 transfected OSRC-2 cells. Presented are representative images (h) of abdominal metastasis viewed by bioluminescent imaging in each group 4 weeks after the orthotopic xenograft transplantation (n=10). Quantitations shown at right (f, h) i A schematic illustration for SMAD4/SMAD7 Signals regulated by miR-452-5p. Data shown are mean±S.D. *p<0.05