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Fig. 6 | Molecular Cancer

Fig. 6

From: Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis

Fig. 6

The identification and verification of circFNDC3B-related downstream genes in BC cells. a Differentially expressed genes in T24 and UM-UC-3 cells after knockdown of circFNDC3B. b Heat map analysis of tumor-related genes identified in this study. Red indicates upregulated expression; green indicates downregulated expression. c and d The possible binding sites of the 5’UTR of G3BP2 with miR-1178-3p were predicted by RegRNA2.0. c Graph of predicted RNA secondary structure. The yellow region indicates the RNAfold predicted structure of the motif. d RNAfold reliability information of pair probabilities. Minimum free energy = − 34.02. e Sequence alignment of miR-1178-3p with circFNDC3B (predicted by Targetscan) and 5’UTR of G3BP2 (predicted by RegRNA2.0). f Immunoblotting of G3BP2, SRC, p-SRC, FAK, p-FAK after knocking down circFNDC3B in T24 and UM-UC-3 cells. g Renilla luciferase activity in UM-UC-3 cells cotransfected with miR-1178-3p mimics and wild-type or mutant G3BP2 5’UTR luciferase reporter. Data are presented as the ratio of Renilla luciferase activity to firefly activity. h Immunoblotting of G3BP2, SRC, p-SRC, FAK, p-FAK in T24 and UM-UC-3 cells transfected with miR-1178-3p mimics or inhibitor. Data are shown as the means±SEM of three experiments. **P < 0.01 (Student’s t-test)

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