Fig. 2

BMP5 is downregulated in sCRC and its low expression correlates with recurrence and poor prognosis. a Representative images of Immunohistochemical staining of BMP5 expression in sCRC (lower) and adjacent normal tissue (upper). BMP5-positive cells mainly located in adjacent benign glands. Four tissue microarrays containing 129 paired samples of CRC and normal colon tissue was assessed by immunohistochemistry. Scale bars represent 50 μm. Sample information: (Left) female, 57, high differentiation; (Right), male, 73, low differentiation. b BMP5 expression analysis in normal colorectal tissue and adenomas (polyps) of GEO datasets (GSE41258, GES81804, GSE71187, and GSE8671), *P < 0.05, ****P < 0.0001. c BMP5 mRNA levels were assessed using TCGA CRC data,*P < 0.05. d Paired samples were selected and analyzed using paired t test, **** P < 0.0001. e Validation group of 28 matched sCRC samples identified BMP5 was significantly decreased in tumor samples (****P < 0.0001, paired t-test). f Correlation analysis of BMP5 expression and patients’ recurrence, *P < 0.05. g Kaplan–Meier analysis of overall survival according to high or low BMP5 expression in adenocarcinomas (Log rank test). h Kaplan–Meier analysis of overall survival according to BMP5 expression and MSI status. (i) mRNA and protein level of BMP5 in colon cell lines. All four cancer cell lines showed downregulated of BMP5 as compared to normal NCM460 cells