Fig. 5
BMP5 supresses migration, invasion, and modulates EMT in CRC. a-b The migration and invasion ability was assessed using Transwell chamber (48 h after transfection for SW480 cells). Representative images were visualized at a magnification of 100×, *P < 0.05, **P < 0.01. c Cell morphological changes in epithelial NCM460 and SW480 cells after knockdown of BMP5. D Western blot showed BMP5 was downregulated in a time course after addition of CoCl2 in SW480 cells. e BMP5 expression could attenuate SW480 cell motility ability induced by CoCl2 mediated EMT. BMP5 and MMP2 expression were validated by blotting. f Pearson correlation between BMP5 and EMT markers in normal colorectal tissue of TCGA samples. g Correlation of fold change (Log2(Tumor/Normal)) between BMP5 and E-cadherin. h E-cadherin mRNA level analysis after transfection of BMP5 WT vector or siRNA, *P < 0.05, **P < 0.01. i E-cadherin protein level analysis after transfection of BMP5 WT vector or infection of BMP5 lentivirus in HT-29 and SW480 cells, as well as siRNA-mediated knockdown in SW480 cells