Fig. 1

piR-36,712 is downregulated in breast cancer and correlated with clinical outcomes in patients. a The top 20 highly expressed piRNAs in breast cancer based on the analysis of TCGA data. b The expression levels of piR-36,712 in breast cancer and paired non-tumor tissues from patients recruited at SYSUCC (Cohort 1) and CHCAMS (Cohort 2). Data are mean ± SEM, ***, P < 0.001). c The expression levels of piR-36,712 in 103 paired breast cancer and non-tumor tissues from TCGA database (mean ± SEM; ***, P < 0.001). d Northern blot of piR-36,712 in breast cancer cells. e piR-36,712 copy number per cell in MCF7 and ZR75–1 cell lines (mean ± SEM). f Distribution of piR-36,712 in cytoplasm and nucleus of breast cancer cells with U6 or GAPDH as nucleus or cytoplasm markers (% ± SEM). g Kaplan-Meier estimates of progression-free survival time in breast cancer patients at SYSUCC (Cohort 1) and CHCAMS (Cohort 2) stratified by piR-36,712 levels in tumor. HR, hazard ratio; CI, confidence interval. h The expression levels of piR-36,712 in breast cancer with (LN ≥ 1) or without (LN = 0) axillary lymph node (LN) metastasis (mean ± SEM; *, P < 0.05)