Fig. 3

piR-36,712 interacts with SEPW1P RNA. a Levels of SEPW1P RNA in MCF7 and ZR75–1 cells with stable overexpression (OE) or knockdown (KD) of piR-36,712 (mean ± SEM; **, P < 0.001; ***, P < 0.0001). b Relative reporter gene activity of psiCHECK2 vector bearing SEPW1P in MCF7 and ZR75–1 cells co-transfected with indicated amount of piR-36,712 mimic or inhibitor (mean ± SEM; **, P < 0.001; ***, P < 0.0001). c Relative reporter gene activity of psiCHECK2 vector bearing SEPW1P with mutation at putative binding site of piR-36,712 in MCF7 and ZR75–1 cells co-transfected with 100 pmol of piR-36,712 mimic. d Schematic diagram of MS2-RNA immunoprecipitation (RIP) assay. e MS2-RIP and qRT-PCR analysis shows interaction of piR-36,712 with SEPW1P RNA in MCF7 and ZR75–1 cells. f Decay of SEPW1P RNA in MCF7 and ZR75–1 cells with piR-36,712 overexpression or knockdown treated with actinomycin D determined by qRT-PCR. g, h RIP and qRT-PCR analysis shows significant increased association with PIWIL1 protein of piR-36,712 (g) and SEPW1P RNA (h) in MCF7 and ZR75–1 cells