Fig. 4

PVT1 regulates HK2 expression by sponging miR-143. (a-b) The effects of si-PVT1, miR-143 inhibitor and si-PVT1 + miR-143 inhibitor on mRNA and protein levels of HK2 in GBC-SD cells. (c-d) The effects of miR-143 mimics, pcDNA-PVT1, and miR-143 mimics+pcDNA-PVT1 on mRNA and protein levels of HK2 in GBC-SD cells. (e-f) Pearson correlation between PVT1 and HK2 mRNA transcript level was measured in GSE76633 and 20 pairs of GBC tissues, respectively. (g-h) Expression level of HK2 was higher in GBC tissues than in normal tissues by IHC staining. Scale bar, 100 μm. (i) Representative HK2 staining patterns. Scale bar, 100 μm. (j-k) High PVT1 expression correlated with advanced TNM stage, metastasis and poor overall survival. (l) The association between PVT1 and HK2 expression. *P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate mean ± SD