Fig. 2

Effects of reactivation of miR-9 by 5-AZA treatment on leukemic potential of EVI1^high AML cell lines and primary AML cells from EVI1^high AML patients. a Analysis of growth of EVI1^high and EVI1^low cell lines after treatment with 5-AZA. Growth was assessed in liquid culture media and cell number and viability assessed using trypan blue staining. Y axis denotes percentage growth inhibition at 3 days. b Representative flow cytometric analysis of apoptosis in EVI1^high and EVI1^low lines treated with 5-AZA. c Histograms depict the frequency of apoptosis in EVI1^high and EVI1^low lines treated with 5-AZA. d Histograms showing the colony-forming ability of EVI1^high and EVI1^low leukemia cell lines treated with 5-AZA. e Representative flow cytometric analysis and f histogram of frequency of apoptosis after primary EVI1 ^high (AML sample H6 and H7) and EVI1^low (AML sample H12 and H13) and CD34 cells were treated with 5-AZA. g Histograms showing the Colony forming ability of EVI1^high, EVI1 ^low primary AML cells and CD34 cells with or without treatment of 5-AZA.All data are representative of two-to-three independent experiments. *, P < 0.05, **, P < 0.01; ***, P < 0.001