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Fig. 3 | Molecular Cancer

Fig. 3

From: RETRACTED ARTICLE: CircPSMC3 suppresses the proliferation and metastasis of gastric cancer by acting as a competitive endogenous RNA through sponging miR-296-5p

Fig. 3

CircPSMC3 directly binds to miR-296-5p and suppresses miR-296-5p activity. a Lysates from MGC803 and AGS cells with circPSMC3 vector were subjected to biotinylation-cirPSMC3 pull down assay, and expression levels of circPSMC3 and miR-296-5p were measured by qRT-PCR. b The Schematic of circPSMC3 wild-type (WT) and mutant (Mut) luciferase reporter vectors. c The relative luciferase activities were analyzed in 293 T cells co-transfected with miR-296-5p mimics or miR-NC and luciferase reporter vectors psiCHECK2-circPSMC3-WT or psiCHECK2-circPSMC3-Mut. d The expressions of miR-296-5p were analyzed by using qRT-qPCR in cells transfected with circPSMC3 or mock vector or si-circ or si-NC vector. e The expression levels of circPSMC3 were determined with qRT-qPCR in cells transfected with miR-296-5p mimics or inhibitor. Data indicate mean ± SD, n ¼ 3. **P < 0.01, ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598