Fig. 4

MiR-296-5p can promote GC cell activities by targeting PTEN. a The Schematic of PTEN 3 UTR wild-type (WT) and mutant (Mut) luciferase reporter vector were shown. The relative luciferase activities were analyzed in 293 T cells co-transfected with miR-296-5p mimics or miR-NC or in-296-5p or in-NC. b The relative expressions of PTEN mRNA were evaluated by using qRT-qPCR in cells transfected with the miR-296-5p mimics or inhibitors respectively. c The relative expressions of PTEN protein were evaluated by using western blot in cells transfected with the miR-296-5p mimics. d The growth curves of cells were measured after transfection with miR-296-5p mimics or inhibitor by using CCK-8 assays. e EdU assays of GC cells transfected with miR-296-5p mimics or miR-NC were performed to evaluate cell proliferation. f Cell motility was examined in cells transfected with miR-296-5p mimics or miR-NC by wound healing assay. g Cell invasion or migration assays were performed in cells transfected with miR-296-5p mimics or miR-NC by using using transwell chamber with or without matrigel respectively. Data indicate mean ± SD of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, Scale bar, 100 mm