Fig. 8

circAKT3 promotes cisplatin resistance of GC cells in vivo. a Xenograft tumors of sacrificed mice with or without CDDP treatment (3 mg/kg, three times a week) at the end of the experiment. b Growth curves of subcutaneous xenograft tumors. c PIK3R1, γH2AX, cleaved caspase-3 and BRCA1 expression levels are shown in representative xenograft tumors by IHC (Left) (400x magnification, scale bars = 50 μm). Quantification of the IHC scores of PIK3R1, γH2AX, cleaved caspase-3 and BRCA1 expression levels (Right). d FISH showing the colocalization of circAKT3 and miR-198 in CDDP-resistant or CDDP-sensitive GC tissues from patients. FISH scores of circAKT3 and miR-198 were further calculated in 14 CDDP-resistant and 30 CDDP-sensitive patient tissues. Nuclei were stained with DAPI. Scale bar, 10 μm. e IHC staining of PIK3R1 in CDDP-resistant or CDDP-sensitive GC tissues from patients (400x magnification, scale bars = 50 μm). The IHC scores of PIK3R1 were further determined in 14 CDDP-resistant and 30 CDDP-sensitive patient tissues. f Three-dimensional scatter plot of circAKT3, miR-198 and PIK3R1 levels in 14 CDDP-resistant and 30 CDDP-sensitive GC tissues from patients. The results are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001