Fig. 5

HIF-1α upregulates Pvt1 expression in G-MDSCs under hypoxic stress. a The mRNA level of HIF-1α in G-MDSCs sorted from spleens and tumor tissues of TB mice was detected using qRT-PCR. G-MDSCs isolated from spleens of TB mice were cultured in an incubator at 37 °C (20% O₂, 5% CO₂) (normoxic conditions) or in a sealed box containing an anaerobic bag to consume oxygen (O₂ < 0.1%, 5% CO₂) (hypoxic conditions). b, c The mRNA and protein levels of HIF-1α were measured via qRT-PCR and western blot (WB) analyses, respectively. d Pvt1 expression was analyzed via qRT-PCR. YC-1, a specific inhibitor of HIF-1α, was used to block hypoxia. e, f HIF-1α and (g) Pvt1 expression in the normoxia, hypoxia, and hypoxia+YC-1 groups were detected via qRT-PCR and WB analyses. ***p < 0.001, **p < 0.01, and *p < 0.05; ns: no significance