Fig. 4

Characterization and roles of exosomes derived from miR-128-3p transfected FHC cells. a Left: exosomes were analyzed under electron microscopy which displayed the same morphology. Scale bar = 200 nm. Right: Nanoparticle tracking analysis were analyzed the size distribution and number of exosomes derived from FHC cells. b Western blotting analysis showing exosome-enriched medium with expression of the exosome marker of CD63 and CD9 and non-expression of the cis-Golgi matrix protein (GM130). c RT-qPCR detection of miR-128-3p expression in exosome derived from FHC cells transfected with Lv-128 and Ctrl. d RT-qPCR analysis of miR-128-3p in the 128-exo cocultured cells were untreated with or treated with RNase A (10 μg/ml) and/or 0.3% Triton X-100 and then further mixed with of RNase inhibitor. e Internalization of exosome derived from FHC-128 cells. Labelled 128-exo (green fluorescent dye, PKH67) were uptake by HCT116OxR (DAPI-labelled) cells. f RT-qPCR analysis of miR-128-3p in recipient HCT116OxR cells that were treated with PBS, NC-exo, 128-exo, 128-exo with anti-control (anti-ctrl) and anti-miR-128-3p (anti-128). g RT-qPCR analysis of miR-128-3p in recipient HCT116OxR cells co-cultured with different incubation time of 128-exo. h RT-qPCR analysis of miR-128-3p in HCT116OxR cells treated with Actinomycin D (ActD) (1 μg/mL) followed by 128-exo treatment for 48 h. Results are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001