Fig. 1

Identification of differentially expressed miRNAs in plasma exosomal sequencing samples. a Venn diagram of differentially expressed miRNAs between CIN I- and other groups (CIN II-III, CC, SCC, and ACC). b, c Principal component analysis (b) and clustering analysis (c) of all 61 significant exosomal miRNAs that were differentially expressed between CIN I- and other groups (CIN II-III, CC, SCC, and ACC). d ROC curves of the top eight significant miRNAs (let-7a-3p, let-7d-3p, miR-30d-5p, miR-144-5p, miR-182-5p, miR-183-5p, miR-215-5p, and miR-4443). ROC analysis was performed to evaluate the sensitivity and specificity of the eight-miRNA signature (i.e. a group of the top eight significant miRNAs) to discriminate CIN II+ from CIN I- subjects. e, f Principal component analysis (e) and clustering analysis (f) of the top eight significant miRNAs. g, h Expression levels and ROC curves of four down-regulated (g) and four up-regulated (h) miRNAs in CIN II+ group compared with those in CIN I- group. Exosomal miRNA expression levels were quantified as RPM in the sequencing data. i Biological pathways enriched for experimentally validated targets by at least five of the top eight miRNAs. Experimentally validated miRNA-target interactions were identified from the miRTarBase database. j miRNA-gene connection network. Circles represent miRNAs. Squares represent experimentally validated target genes by at least three of eight miRNAs. The pink, blue, and green squares represent target genes that were involved in < 5, 5–10, and > 10 significant pathways, respectively