Fig. 5

Knockdown of miR-203a-3p reverses sh-circTADA2A-induced the attenuation of cell migration, invasion and proliferation in OS cells. a HOS and 143B cells were stably transfected with N.C. or sh-circTADA2A or cotransfected with sh-circTADA2A and miR-203a-3p sponge. The migration and invasion ability was evaluated by Transwell migration and Matrigel invasion assay. Scale bars, 100 μm. b Wound-healing assay demonstrated the reversion of migration ability by miR-203a-3p sponge. Representative images are shown. c Colony formation assay demonstrated the effect of sh-circTADA2A and the miR-203a-3p sponge on the colony-forming ability of OS cells. d Proliferation of OS cells transfected with control N.C. and sh-circTADA2A with or without the miR-203a-3p sponge was evaluated by a CCK-8 assay. e Representative images of soft agar colony formation assay demonstrated the proliferation ability of stable OS cells at 0, 7, and 14 days. Scale bars, 100 μm. f The effects of circTADA2A knockdown and miR-203a-3p sponge rescue on circTADA2A silence were evaluated by apoptosis assay. Apoptotic rates were determined by Annexin V-FITC/PI staining. Data are from three independent experiments (mean ± SEM) (a-d and f) or are representative of three independent experiments with similar results (e) (*P < 0.01 vs control or as indicated by Student’s t-test)