Fig. 7
From: Novel lncRNA-IUR suppresses Bcr-Abl-induced tumorigenesis through regulation of STAT5-CD71 pathway
Overexpressing CD71 reverses the inhibitory effect of lncRNA-IUR-5 on cell survival and tumorigenesis of Abl transformants. a, Western blotting analysis of CD71 levels in K562 cell lines overexpressing CD71 and control. b, Cell viability of indicated K562 cell lines was analyzed by flow cytometry after treatment with indicated concentrations of imatinib for 36 h (n = 3; means ± SEM; *p < 0.05). c, Shown were representative images of nude mice subcutaneously injected with indicated K562 cell lines, and tumors were excised from these nude mice. d, Scheme of plasmids overexpressing lncRNA-IUR-5 alone (IUR-5), lncRNA-IUR-5 with CD71 (IUR-5-CD71), and EV. e, Western blotting and RT-PCR were performed to examine the protein and mRNA levels as indicated. f, Cell viability of indicated K562 cell lines were analyzed by flow cytometry after treatment with indicated concentrations of imatinib for 36 h (n = 3; means ± SEM; *p < 0.05). g and h, Shown were representative images of nude mice subcutaneously injected with indicated K562 cell lines. Tumors were excised from nude mice (g), and tumor growth was analyzed by bioluminescence imaging (h). Shown were representative images from at least three independent experiments with similar results