Fig. 6
The m6A modification is involved in the upregulation of RP11 in CRC cells. a m6A RIP-qPCR analysis of RP11 in HCT-15, HCT-8 and NCM460 cells. b After transfection with vector control or ppB/Mettl3 for 24 h, RP11 expression was measured by qRT-PCR. c After transfection with vector control or pcDNA/Alkbh5 for 24 h, RP11 expression was measured by qRT-PCR. d After transfection with vector control or ppB/Mettl3 for 24 h, HCT-15 cells were further treated with Act-D for the indicated times, and RP11 expression was measured by qRT-PCR. e After transfection with vector control or ppB/Mettl3 for 24 h, the cytoplasmic, nuclear, and chromatin fractions of HCT-15 cells were separated for RNA extraction and qRT-PCR. f After transfection with vector control or ppB/Mettl3 for 24 h, binding between RP11 and hnRNPA2B1 in HCT-15 and HCT-8 cells was analysed by RIP-PCR using an antibody against hnRNPA2B1. Data are presented as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 compared with control