Fig. 2
From: MED12 exerts an emerging role in actin-mediated cytokinesis via LIMK2/cofilin pathway in NSCLC
MED12 knockout suppressed cell proliferation and xenograft tumor growth. a DNA sequencing validated the indels genotype of two MED12 alleles in PC9 MED12 knockout clones. b Western blotting assay of MED12 in PC9 single clones after using specific MED12 sgRNAs by CRISPR. c-d Western blotting assays of MED12 in WT, MED12 KO and MED12 reconstitution single clones of PC9 and SPC-A1 cells. e MTT assay showed the proliferation ability of WT, MED12 KO and MED12 reconstitution single clones of PC9 and SPC-A1. f Colony formation assays in WT, MED12 KO and MED12 reconstitution single clones of PC9 and SPC-A1 cells. g-j Efficient knockdown of MED12 in various NSCLC cell lines were revealed by western blotting and cell proliferation examined by MTT assay. k Schema of xenograft in vivo assay. l Tumor growth curve of WT/KO1 cells (left panel) and KO1/KO1-MED12 cells (right panel) in nude mice. The number of mice in each group was eight. m Image of xenografts when nude mice sacrificed. n Tumor weight of WT/KO1 group (left panel) and KO1/KO1-MED12 group (right panel)