Fig. 5

MED12 knockout altered the expression of actin-relevant genes in NSCLC cells. a Heat map of mRNA changes in WT and MED12 KO single clones of PC9 by bulk RNA-seq. b Venn diagrams depicted 751 common differential genes in MED12 KO single clones of PC9. c KEGG pathway assay of differential mRNA transcripts in MED12 KO clones identified by RNA-seq. d Heat map of mRNA changes in actin cytoskeleton by RNA-seq. e Representative images of single cell sorted from WT and MED12 KO1 of PC9 cells. Scale bar: 100 μm. f Heat map of mRNA changes in single cells sorted from WT and MED12 KO1 of PC9 cells by Single cell RNA-seq. g Venn diagrams depicted 107 common differential genes in bulk RNA-seq and Single cell RNA-seq. h Real-time PCR assay of LIMK2 in WT, MED12 KO1 and KO1-MED12 clones of PC9 and SPC-A1 cells