Fig. 1
TGIF1 expression in PDAC and TGIF1 ablation in the pancreas do not interrupt pancreatic development in mice. a IHC (i) and western blot (ii) analyses of TGIF1 protein expression in mouse normal pancreata, PanINs, and PDAC tissues from mice with indicated genotypes. b i. TGIF1 expression in the human PDAC tissue microarrays (TMAs), as shown by IHC analysis. ii. Kaplan-Meier survival curves of low and high expression TGIF1 groups. *P < 0.05. c Schematic indicating the targeted TGIF1 allele before and after Cre-mediated recombination. d PCR genotyping reactions detected the wild-type and TGIF1 loxp alleles from the tail DNA of mice littermates. e Gross anatomy of the pancreas in wild-type and Pdx-1CreTGIF1L/L mice. f Glucose tolerance test (GTT) determined the blood glucose concentration in wild type and Pdx-1CreTGIF1L/L mice. n.s stands for not statistically significant. g Representative images of hematoxylin and eosin (H&E) staining and immunostaining detection of the epithelial marker cytokeratin19 (CK19), islet enzymes insulin and glucagon, acinar marker amylase and Alcian blue stain in the pancreata of wild-type and Pdx-1Cre TGIF1L/L mice (insert 400 x). Scale bar = 100 μm