Fig. 3
Conditional TGIF1 deletion promotes the development of highly metastatic PDAC in KrasG12D P53L/L models. a i, The autopsy image of a 7-week-old PKTP mouse. ii, Gross images of multi- organ metastasis in the PKTP mcie (arrows: tumor nodule). iii, Histological H&E staining analysis of PDAC and metastatic nodules in secondary disseminating organs of the PKTP mice reveals focal neoplasm lesions with PDAC histology. b Kaplan–Meier survival curves of the PKP and PKTP mice (P < 0.01). c Alcian blue, modified Mason’s stain and IHC staining for CK19, fibronectin, α-tubulin, E-cad, TGFβ1, p-Smad2, p-STAT3, pAkt, p44/42, VEGFA, VWF and CD31 in PDAC from the PKP and PKTP mice. Scale bar, 100 μm