Fig. 6

cDNA microarray analysis compared the differential gene expression of murine primary PDAC cells derived from PKP and PKTP mice. a Heat map showing the changes in gene expression in response to TGIF1 loss in PDAC. P < 0.05 was considered significantly differential gene expression. The scale bar extends the expression level of the gene from the florescence ratios of 2.43–12.55 Red, high expression level compared with the means. Green, low expression level compared with the means. Heatmap of the selected top 10 genes that were upregulated or downregulated in TGIF1-null PDAC cells as compared with TGIF1-sufficient PDAC cells. b RT qPCR analysis confirmed that the mRNA levels of IFITM3, ETV1, HAS2, and RHOJ were upregulated; whereas TFF1, MUC5AC, AVI1 andTFF2 were downregulated in PKTP cells, compared with PKP cells. The data were normalized to glyceraldehyde 3-phosphate dehydrogenase. Results are presented as mean ± SD of triplicates **p < 0.01; ***p < 0.001. c Western blot assays were performed to demonstrate the increased Etv1, Has2, RhoJ and Acta2 protein expression in PKTP cells as compared with the PKP cells. d Immunocytochemical detection of Etv1 and HAS2 expression in in PKTP cells as compared with the PKP cells. e IHC examination with antibodies against ETV1, HAS2, RhoJ and CD44 confirmed the increased expression of ETV1 and HAS2, RhoJ and CD44 in TGIF1-deficient PDAC as compared to TGIF1-proficient PDAC. Representative images are shown (× 500)