Fig. 4
From: miR-301a promotes lung tumorigenesis by suppressing Runx3
Inhibition of lung tumorigenesis in miR-301a−/−;KrasLA2 mice correlates with elevated Runx3 expression. (a) Venn diagram of predicted miR-301a target genes based on analysis of 6 databases (shown in bold). (b) Venn diagram of specific genes between predicted miR-301a target genes from (a) and DEGs identified by RNA-Seq. (c) Real-time PCR validation of 6 significantly upregulated genes from (b) (n = 3 per group). (d) IPA interaction network analysis of significant DEGs identifies the Runx3/β-catenin pathway in lung tumorigenesis with miR-301a deletion. (e) Western blotting analyses of Runx3 and β-catenin in the lung. Lung tissues were harvested from KrasLA2 (n = 4) and miR-301a−/−;KrasLA2 (n = 5) mice at 9 weeks of age. (f) Quantification of the expression of Runx3 and β-catenin from (e) using Image J with β-actin as a reference. (g) Immunofluorescence analyses of Runx3 and β-catenin in the lung. Lung tissues were from (e) and lung sections were stained with antibodies against Runx3 and β-catenin and DAPI (nucleus). Scale bars = 50 μm. Value represented the mean ± s.d. of three independent experiments. ** P < 0.01 indicated a significant difference between KrasLA2 and miR-301a−/−KrasLA2 mice (two-tailed, unpaired Student’s t-test in c and f)