Fig. 3

CircACVR2A serves as a miR-626 sponge in BC cells. a Eight potential target miRNAs of circACVR2A were predicted by CircInteractome and miRanda. (b)Schematic model showed the putative binding sites of eight miRNA candidates associated with circACVR2A. c Relative levels of circACVR2A in T24 and UM-UC-3 lysates after RNA pull down using circACVR2A probe or oligo probe. d Relative levels of eight miRNAs in T24 and UM-UC-3 lysates pulled down by circACVR2A probe or oligo probe. e Relative levels of circACVR2A in T24 and UM-UC-3 lysates captured by biotinylated wild-type miRNA-626 (Biotin-miR-626-wt) or mutant miR-626(Biotin-miR-626-mut). f Luciferase reporter assay in HEK293T cells co-transfected with miRNA mimics, psiCHECK-2-wild type circACVR2A (circACVR2A-wt) or psiCHECk-2-mutant type circACVR2A (circACVR2A-mut) plasmids. g Schematic of circACVR2A wild-type (wt) and mutant (mut) luciferase reporter vectors. h FISH showed the co-localization between circACVR2A and miR-626 in T24 cells. Nuclei were stained with DAPI. CircACVR2A was labeled with Cy3 and miR-626 was labeled with Cy5