Fig. 4

Up-regulated miR-626 promotes cell proliferation, migration and invasion through targeting EYA4 in vitro. a Relative expression of miRNA-626 in SV-HUC-1, T24 and UM-UC-3 cell lines. b Cell proliferation ability of T24 and UM-UC-3 cells transfected with mimics NC or miR-626 mimics was evaluated by MTS assay. c Cell migration capability of T24 and UM-UC-3 cells transfected with mimics NC or miR-626 mimics was evaluated by wound healing assays. d Cell proliferation ability of T24 and UM-UC-3 cells transfected with mimics NC or miR-626 mimics was evaluated by colony formation assay. e-f The influence on cell migration and invasion abilities of T24 and UM-UC-3 cells transfected with mimics NC or miR-626 mimics was assessed by transwell migration and matrigel invasion assays. g Luciferase reporter assay in HEK293T cells co-transfected with miRNA mimics, psiCHECK-2-wild type EYA4 (EYA4-wt) or psiCHECk-2-mutant type EYA4 (EYA4-mut) plasmids. h Schematic of EYA4 wild-type (wt) and mutant (mut) luciferase reporter vectors. i Western blot analysis indicated that miR-626 could down-regulate EYA4 and up-regulate ID2 expression in BC cells