Fig. 3
From: Lung adenocarcinoma-intrinsic GBE1 signaling inhibits anti-tumor immunity
IFN-I pathway is required for the effect of GBE1 knockdown on CCL5 and CXCL10 upregulation. a DAVID analysis of the top 20 altered pathways using KEGG in shGBE1 A549 cells compared to control. The correlation analysis of TCGA data for IFN response signature and CD8 (b) or CCL5, CXCL10 (c) expression. d Heatmap of relative mRNA expression for IFNα/β-signaling pathway core-enriched genes in shGBE1 A549 cells compared to control. e Quantification of IFNα/β pathway core-enriched genes differentially expressed in shGBE1 A549 cells compared to control. f The real-time PCR analysis of mRNA expression of IFNα/β pathway in shGBE1 A549 and control cells. g The real-time PCR analysis of the core-enriched genes differentially expressed by the indicated poly(I:C) in A549 cells. Real-time PCR (h) and ELISA (i) analysis of CCL5 and CXCL10 before and after treatment with poly(I:C) in shNC and shGBE1 A549 cells