Fig. 4

circTRIM33–12 upregulates TET1 expression in HCC cells. a-d The relative mRNA and protein levels of TET1 were detected in SMMC-7721 and Huh 7 cells after transfection with circTRIM33–12, circTRIM33–12 shRNA, or the control using qRT-PCR and western blotting, respectively. e The luciferase activity of luc-TET1 or mutant luc-TET1 in Huh 7 cells after cotransfection with circTRIM33–12 shRNA or circTRIM33–12 and miR-191 siRNA. f The luciferase activity of luc-TET1 or mutant luc-TET1 in SMMC-7721 cells after cotransfection with circTRIM33–12 or the mock control. g and h The expression of TET1 in 200 cases of HCC tissues was detected by qRT-PCR and IHC. A positive correlation between circTRIM33–12 and TET1 was observed in tumor tissues at the protein (R2 = 0.0441; P = 0.0028) and mRNA (R2 = 0.04751; P = 0.0019) levels. The data are represented as the mean ± SD, n = 3. *P < 0.05; NS, no significant