Fig. 4

UCA1 interacts with miR-193a and miR-214, and then modulate PDL1 expression. a predicted interaction between UCA1 and miR-193a or miR-214. b dual luciferase assay. HEK293T cells were co-transfected with reporter vector using full sequence of UCA1 as 3’UTR of firefly luciferase, and miRNAs mimics or inhibitors. The luciferase activity was detected 48 h post transfection. c The relative miR-193a and − 214 expression was determined by qRT-PCR. d predicted interaction between PDL1 and miR-193a or miR-214. e dual luciferase assay. HEK293T cells were co-transfected with PDL1 3’UTR reporter vector and the mimic or inhibitor of miR-193a or miR-214 for 48 h. Luciferase activities were detected and results were analyzed by student’s t-test. f miR-193a and miR-214 repress endogenous PDL1 expression