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Fig. 6 | Molecular Cancer

Fig. 6

From: METTL3 promote tumor proliferation of bladder cancer by accelerating pri-miR221/222 maturation in m6A-dependent manner

Fig. 6

miR221 and miR222 targeted PTEN in bladder cancer. a. Predictive miR221/miR222 binding sites in the 3′-UTR of PTEN mRNA. b. Dual luciferase reporter assays demonstrated that PTEN was direct target of miR221/222 (*P < 0.05). c and d. PCR and western blot analysis showed that knockdown of METTL3 increased the PTEN expression in T24 cells, while overexpression of METTL3 in T24 cells decreased the PTEN expression. e and f. Western blot analysis showed that miR221 and miR222 mimics could partly decrease the protein expression level of PTEN induced by knockdown of METTL3 (e), while miR221 and miR222 inhibitors could partly increase the protein expression level of PTEN induced by overexpression of METTL3 in EJ and T24 cells (f)

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Molecular Cancer

ISSN: 1476-4598