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Fig. 3 | Molecular Cancer

Fig. 3

From: Discovery of a novel long noncoding RNA overlapping the LCK gene that regulates prostate cancer cell growth

Fig. 3

Characterization of HULLK. a HULLK is transcribed from the sense strand of DNA. LNCaP cells were seeded for 48 h and treated with 1 nM R1881 for 24 h. RNA was collected and cDNA was synthesized using strand-specific primers. qPCR was performed with two independent LCK primer sets that amplify HULLK, n = 3. b HULLK is localized to the cytoplasm. LNCaP cells were seeded for 48 h and treated with 1 nM R1881 for 24 h. Whole cell lysates were separated into cytoplasmic and nuclear fractions by centrifugation. RNA was collected from each fraction and cDNA was synthesized using the iScript cDNA synthesis kit. qPCR was performed with two independent LCK primer sets that amplify HULLK, and cytoplasmic and nuclear control genes, n = 3

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Molecular Cancer

ISSN: 1476-4598