Fig. 1

circSLC8A1 is significantly down-regulated in bladder cancer tissues and cell lines. a Schematic illustration showing the circularization of SLC8A1 exon 1 forming circSLC8A1. The existence of circSLC8A1 was proved by RT–PCR and its back splicing junction was verified by Sanger sequencing. Red arrow indicates the special splicing junction of circSLC8A1. b and c qRT-PCR assay with divergent primers confirmed the low expression of circSLC8A1 in 70 pairs of human bladder cancer tissues compared with their adjacent normal tissues. d The expression of circSLC8A1 in SV-HUC-1 and bladder cancer cell lines were measured by qRT-PCR. e The existence of circSLC8A1 was validated in 5637 and T24 cell lines by RT–PCR. Divergent primers amplified circSLC8A1 in cDNA but not genomic DNA (gDNA). GAPDH was used as negative control. f The expressions of circSLC8A1 and SLC8A1 mRNA in 5637 and T24 cells treated with or without RNase R were detected by qRT-PCR. The relative levels of circSLC8A1 and SLC8A1 mRNA were normalized to the value measured in the mock treatment. Data are mean ± SD, n = 3