Fig. 4

Lnc34a interacts with epigenetic regulators (n = 3). The interactions between Lnc34a and PHB2, DNMT3a, and HDAC1 were assessed by western blot following an RNA pull-down (a) and RIP assays (b) with HCC-LM3 cell. c RIP showing that the silencing of DNMT3a does not impact the interaction between Lnc34a with PHB2 or HDAC1. d RIP showing that the silencing of PHB2 disrupts the interaction between Lnc34a and DNMT3a, but has no effect on the interaction of Lnc34a with HDAC1. e RIP showing that the silencing HDAC1 has almost no effect on the interaction between Lnc34a with DNMT3a and a rare effect on the interaction of Lnc34a with PHB2. f PHB2 and HDAC1 were examined by western blot following RNA pull-down in samples with the different Lnc34a probe fragments and input samples. g-i Silencing the expression of DNMT3a (g), HDAC1 (h), and PHB2 (i) increased miR-34a expression levels in HCC-LM3 cells. j-l The expression level of miR34a was examined by qRT-PCR in HCC-LM3 cells treated with HDAC inhibitor SAHA (j) or TSA (k) and DNA methylation inhibitor 5-Aza-CdR (l). Three independent experiments were performed in the above assays. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001