Fig. 6

Restoration of NR2F6 rescues circRHOT1 deletion-mediated suppression of HCC growth and metastasis. a-c NR2F6 mRNA levels were upregulated in HCC tissues compared with those in adjacent normal tissues according to GSE36376 dataset (a), GSE25097 dataset (b) and qRT-PCR results (c). d, e Immunohistochemical analysis (d) and western blotting (e) indicated that the NR2F6 protein level is upregulated in HCC tissues. f Kaplan–Meier survival analyses were conducted to assess the prognostic significance of NR2F6 expression in HCC patients. g The NR2F6 protein level was restored by transduction with NR2F6 ectopically expressing plasmid in circRHOT1-knockout HCC cells. h-j The effects of NR2F6 on HCC cell proliferation (h), migration (i) and invasion (j) were assessed by CCK-8 and Transwell assays. k The effect of NR2F6 on tumor growth in vivo was determined by xenograft assays. *p < 0.05, **p < 0.01 and ***p < 0.001. All the data are representative of at least three independent experiments and presented as the means ± SD