Fig. 2

WTAP promotes tumor growth of HCC cells in vitro and in vivo. a, b and c Negative control or siRNA (si-WTAP #1, #2) was transfected into Huh7 (a), Hep3B (b) and PLC/PRF/5 (c) cells, respectively. The knockdown efficiency was tested and proliferation capacities were detected by CCK-8 and colony formation assay, with bar charts showing colony numbers; d EdU assay was applied to compare the cell proliferation ability in Huh7 and Hep3B (scale bar, 100 μm); e, f and g Tumor growth curve (f) of stable WTAP silenced PLC/PRF/5 cells (or negative control) in the xenograft mouse model was based on tumor size measurement. Moreover, tumor nodules (e) were collected and tumor weights (g) were recorded to present the growth difference within the influence of WTAP. h, i and j Tumor growth curve (i) of stable WTAP overexpressing HCCLM3 cells (or negative control) in the xenograft model was presented, followed by the collection of tumor nodules (h) and tumor weight records (j)