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Fig. 7 | Molecular Cancer

Fig. 7

From: Circular RNA circ-ZKSCAN1 inhibits bladder cancer progression through miR-1178-3p/p21 axis and acts as a prognostic factor of recurrence

Fig. 7

Circ-ZKSCAN1 downregulates p21(CDKN1A) expression by partially sponging miR-1178-3p. a Differentially expressed genes between the Negative Control (NC) and siRNAs groups were showed in the heatmap. Red indicates upregulated expression; green indicates downregulated expression. b-c qPCR and western blotting showed that enforced circ-ZKSCAN1(cZK) increased the expression of p21(CDKN1A) at both the mRNA and protein levels in UM-UC-3 and T24. d The schematics revealed that circ-ZKSCAN1(cZK) and p21 shared the same binding sites of miR-1178-3p. e Western blotting showed that p21 was downregulated in UM-UC-3 and T24 after transfection of miR-1178-3p mimics. f The dual-luciferase reporter assays revealed that luciferase activities was decreased in HEK293T cells after co-transfection with miR-1178-3p mimics and p21-wild-type (p21-wt) vector or p21-mutant (p21-mut) vector. g p21 protein expression levels were detected by western blotting after transfected miR-1178-3p mimics or co-transfected miR-1178-3p and circ-ZKSCAN1(cZK). The abbreviations of circ-ZKSCAN1, miR-1178-3p mimics and CDKN1A are cZK, miR-1178 and p21, respectively

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Molecular Cancer

ISSN: 1476-4598