Fig. 3

SNS-032 restrains outgrowth of xenografted UM cells and UM patient-derived xenograft (PDX) in NOD-SCID mice. a-e The effects of SNS-032 on xenografted Omm1 cells. UM xenografts were administrated with vehicle or SNS-032 for 14 days. a Tumor size measured every other day versus time was plotted. *, P < 0.05; ***, P < 0.001, Student’s t test. b Representative images of tumors removed from mice of each group are shown. c SNS-032 significantly reduced tumor weights dissected on day 15 after administration. ***, P < 0.001, Student’s t test. d H&E and IHC staining of tumor sections with anti-Ki67 and –active caspase-3 from mice of each group are shown. Scale bar: 100 μm. e SNS-032 inhibited CTD phosphorylation of RNA Pol II at Ser2, Ser5 and Ser7 sites; the expression and phosphorylation at Ser127 of YAP; as well as the expression of apoptosis-related protein Survivin and the stemness-related proteins KLF4 and Slug in tumor tissues detected by Western blot analysis with the indicated antibodies. f-j The effects of SNS-032 on UM PDX model. MP41 xenografts were administrated with vehicle or SNS-032 for 30 days. f-h The growth curves of MP41 PDX were shown, and the tumors were removed, photographed and weighed. **, P < 0.01; ***, P < 0.001, Student’s t test. i Tumor sections were performed H&E and IHC staining with anti-Ki67. Scale bar: 100 μm. j Cell lysates from the indicated xenografts were subjected to Western blot analysis