Fig. 5
From: Exosomal miR-21 regulates the TETs/PTENp1/PTEN pathway to promote hepatocellular carcinoma growth
Prediction of the miR-21 target genes. a miR-21 target sites in the conservative sequence of TET1, TET2 and TET3. b The luciferase expression level of HCC cells transfected with TET1, TET2 or TET3 wild-type 3′ UTR vector (WT) or mutant 3′ UTR vector (Mu, miR-21 target sites were mutated) and miR-21 mimics. c Hep3B cells were transfected with miR-21 inhibitors (miR-21 I) or miR-21 mimics, then Exo-normal or Exo-SNU-449 (Exo-S) were added to the cells, and TET1, TET2 and TET3 expression was measured by real-time qPCR analysis. d and e After different treatments, TET1, TET2 and TET3 expression was measured by western blot analysis. Each bar represents the mean ± SD determined from three samples (*P < 0.01, vs. control)