Fig. 6

MiR-21 targets TETs to regulate the expression of PTENp1 and PTEN. a and b Hep3B cells were transfected with miR-21, miR-21 + TET1-O, miR-21 + TET2-O, miR-21 + TET3-O, or miR-21 inhibitors. Then, PTEN and PTENp1 expression was measured by real-time qPCR analysis (a), and western blot analysis was used to detect the expression of PTEN (b). c and d Hep3B cells were transfected with TET1-O, TET2-O, TET3-O or miR-21 inhibitors (miR-21 I), and then Exo-normal or Exo-SNU-449 (Exo-S) were added to the cells. PTEN and PTENp1 expression was measured by real-time qPCR analysis (c), and PTEN expression was measured by western blot analysis (d). e Hep3B cells stably infected with miR-21 or miR-21 inhibitor lentivirus were subjected to methylation-specific PCR assay. f The 5hmC level at the PTENp1 promoter upon miR-21 overexpression was analyzed using glucMS-qPCR. The values expressed are the mean ± SD from three samples (^*P < 0.01, vs. control)