Fig. 5

Effects of circFOXP1 on PTBP1, PKM1, PKM2 and PKLR expression in GBC cells. a, b The relative protein expression of PTBP1 after transfected with pLCDH-vector and pLCDH-circFOXP1 in NOZ and SGC-996 cells or sh-NC, sh-circFOXP1–1 and sh-circFOXP1–2 in NOZ cell were determined by immunofluorescence assays, original magnification, 200×. c The mRNA expression of PKM1 and PKM2 were determined with qRT-PCR after transfection of NOZ (top) and SGC-996 cells (low) with sh-NC, sh-circFOXP1–1 or sh-circFOXP1–2. d The relative protein expression of PKM1 or PKM2 after transfected with sh-NC, sh-circFOXP1–1 or sh-circFOXP1–2 in NOZ and SGC-996 cells was determined by western blot analysis (n = 3). e, f The mRNA and protein expression of PKLR were determined with qRT-PCR and western blot analysis after transfection of NOZ and SGC-996 cells with sh-NC, sh-circFOXP1–1 or sh-circFOXP1–2 or GBC-SD cells with pLCDH-vector and pLCDH-circFOXP1. All data are shown as mean ± S.E.M., n = 3 or more, **P < 0.01