Fig. 7

circFOXP1 promotes PKLR expression by binding to miR-370 in GBC cells. a MiR-370 have complementary base pairing with circFOXP1 using circRNAs from RNA sequencing targeted miRNAs predicted by miRanda (www.microrna.org) and the online software tools circinteractome (http://circinteractome.nia.nih.gov) (left). The wild type and mutant type complementary sequences of the circFOXP1 and miR-370 binding sequence are shown (right). b Luciferase reporter assays were performed in NOZ cells co-transfected with miR-370 mimic or miR-NC and circFOXP1-WT1/2 or circFOXP1-MUT1/2 reporter plasmids. Data are shown as mean ± S.E.M., n = 3, **P < 0.01. c The wild-type and mutant-type complementary sequences of the PKLR and miR-370 binding sequence are shown. d Luciferase reporter assays were performed in NOZ and SGC-996 cells transfected with pLCDH-vector, pLCDH-circFOXP1, miR-370 mimic or co-transfected with miR-370 mimic+pLCDH-circFOXP1 and PKLR-WT or PKLR-MUT reporter vector. Data are shown as mean ± S.E.M., n = 3, *P < 0.05, **P < 0.01, n.s., not significant. e The relative expression of PKLR was detected after transfection of NOZ and SGC-996 cells with sh-NC, sh-circFOXP1–1 or sh-circFOXP1–2, miR-370 inhibitor or cotransfection with miR-370 inhibitor and sh-circFOXP1–1. f The relative expression of PKLR was detected after transfection of GBC cells with pLCDH-vector, pLCDH-circFOXP1 or cotransfection with pLCDH-circFOXP1 and miR-370 mimic. g Proposed mechanism by which circFOXP1 promoted GBC progression by interacting with PTBP1 or sponging miR-370 targeting PKLR. Data are shown as mean ± S.E.M., n = 3 or more, **P < 0.01, n.s., not significant