Fig. 3

YTHDF2 inhibits IL-11 and Serpin E2 expression in HCC cells. (a) Volcano plots showing up (red) - or down (blue) -regulated genes in SMMC7721-shYTHDF2 versus SMMC7721-shCtrl cells, as assessed by RNA-seq. (b) GO analysis based on the RNA-seq in (A), showing the most significant GO terms and the P values. (c) Screening strategy showing a group of overlapped genes that were concomitant in YTHDF2-deficiency-induced upregulation and hypoxia-induced hyper-upregulation. (d, g) Immunoblots showing STAT3 phosphorylation (d) and Serpin E2 (g) expression in indicated SMMC7721 cells grown for 24 h under Nx or Hx. n = 2 independent experiments. (e) RT-qPCR analysis of the relative mRNA levels of IL11 (left) and SERPINE2 (right) in indicated SMMC7721 cells grown for 24 h under Nx or Hx. n = 3 biological replicates. (f) ELISA analysis of the quantitative protein levels of IL-11 in the culture supernatant of indicated SMMC7721 cells grown for 24 h under Nx or Hx. n = 3 biological replicates. (h) Volcano plots showing up (red) - or down (blue) -regulated genes in SMMC7721-OE versus SMMC7721-EV cells grown for 12 h under hypoxia, as assessed by RNA-seq. (i) RT-qPCR analysis of the relative mRNA levels of IL11 (left) and SERPINE2 (right) in SMMC7721-EV and SMMC7721-OE cells grown for 0, 6, 12 or 24 h under Hx. n = 3 biological replicates. (j) Immunoblots showing STAT3 phosphorylation in indicated SMMC7721 cells grown for 24 h under Nx or Hx. n = 2 independent experiments. Error bars indicate means ± SEM *P < 0.05, **P < 0.01, ***P < 0.001, **** P < 0.0001. P-values were determined by two tailed t-test