Fig. 2

m⁶A methylation inhibited ocular melanoma tumorigenesis. a A colony formation assay was performed to assess the tumor growth of PIG1 cells with or without METTL3 knockdown. b Statistics of visible colonies in colony formation assays performed using PIG1 cells with or without METTL3 knockdown. The colony number of the control group was set to 1. All of the experiments were performed in triplicate, and relative colony formation rates are shown as the mean ± SEM. n = 3, ***p < 0.001. c GSEA plots evaluating the changes in apoptosis, cell differentiation and cell death in normal melanocyte cells with or without METTL3 knockdown. NES, normalized enrichment score. d A colony formation assay was performed to assess the tumor growth of ocular melanoma cells with or without ALKBH5 knockdown. e Statistics of visible colonies in colony formation assays performed using ocular melanoma cells with or without ALKBH5 knockdown. The colony number of the control group was set to 1. All of the experiments were performed in triplicate, and relative colony formation rates are shown as the mean ± SEM. n = 3, **p < 0.01. f A transwell assay was performed to evaluate the migratory ability of ocular melanoma cells with or without ALKBH5 knockdown. g Statistics of cells in the transwell assay performed using ocular melanoma cells with or without ALKBH5 knockdown. The value obtained for the control group was set to 1. All of the experiments were performed in triplicate, and relative metastasis rates are shown as the mean ± SEM. n = 3, *p < 0.05, **p < 0.01. h GSEA plots evaluating the changes in apoptosis, cell differentiation and cell death in ocular melanoma cells with or without ALKBH5 knockdown. NES, normalized enrichment score